Rlp24 activates the AAA-ATPase Drg1 to initiate cytoplasmic pre-60S maturation

• DOI: 10.1083/jcb.201205021
• Publication Date: 2012-11-26T21:25:19Z
• Authors: Lisa Kappel; Mathias Loibl; Gertrude Zisser; Isabella Klein; Gernot Fruhmann; Christof Gruber; Stefan Unterweger; Gerald Rechberger; Brigitte Pertschy; Helmut Bergler
• Keywords: AAA proteins; ribosome biogenesis; Eukaryotic Ribosome

Formation of eukaryotic ribosomes is driven by energy-consuming enzymes. The AAA-ATPase Drg1 essential for the release several shuttling proteins from cytoplasmic pre-60S particles and loading late joining proteins. However, its exact role in ribosome biogenesis has been unknown. Here we show that protein Rlp24 recruited to stimulated ATPase activity. ATP hydrolysis second AAA domain was required In vitro, specifically exclusively extracted purified particles. promoted interaction with nucleoporin Nup116. Subsequent first dissociated Rlp24, liberating both consecutive cycles Our results defines a key event large subunit formation prerequisite progression maturation.