Coordinated waves of actomyosin flow and apical cell constriction immediately after wounding

• DOI: 10.1083/jcb.201211039
• Publication Date: 2013-07-22T15:56:35Z
• Authors: Marco Antunes; Telmo Pereira; João V. Cordeiro; Luis Almeida; Antonio Jacinto
• Keywords: Actin remodeling; Apical constriction; Calcium in biology

Epithelial wound healing relies on tissue movements and cell shape changes. Our work shows that, immediately after wounding, there was a dramatic cytoskeleton remodeling consisting of pulse actomyosin filaments that assembled in cells around the edge flowed from to toward margin wound. We show this flow regulated by Diaphanous ROCK it elicited wave apical constriction culminated formation leading cable, structure is essential for closure. Calcium signaling played an important role process, as its intracellular concentration increased dramatically down-regulation transient receptor potential channel M, stress-activated calcium channel, also impaired flow. Lowering activity Gelsolin, known calcium-activated actin filament-severing protein, response, indicating cleaving existing filament network part process.