Two appendages homologous between basal bodies and centrioles are formed using distinct Odf2 domains

• DOI: 10.1083/jcb.201303071
• Publication Date: 2013-11-05T04:05:59Z
• Authors: Kazuhiro Tateishi; Yuji Yamazaki; Tomoki Nishida; Shin Watanabe; Koshi Kunimoto; Hiroaki Ishikawa; Sachiko Tsukita
• Keywords: Ciliogenesis; Basal body; Centriole; Appendage

Ciliogenesis is regulated by context-dependent cellular cues, including some transduced through appendage-like structures on ciliary basal bodies called transition fibers and feet. However, the molecular basis for this regulation not fully understood. The Odf2 gene product, ODF2/cenexin, essential both ciliogenesis formation of distal subdistal appendages centrioles, which become bodies. We examined effects deletion constructs in Odf2-knockout F9 cells. Electron microscopy revealed that fiber required ODF2/cenexin fragment containing amino acids (aa) 188–806, whereas foot aa 1–59 188–806. These sequences also formed appendages, respectively, indicating centriole are molecularly analogous to those used differential study incorporation function molecules associated with each appendage type. found were stabilized centrosomal microtubules.