Interdomain allosteric regulation of Polo kinase by Aurora B and Map205 is required for cytokinesis

• DOI: 10.1083/jcb.201408081
• Publication Date: 2014-10-21T04:13:40Z
• Authors: David Kachaner; Xavier Pinson; Khaled Ben El Kadhi; Karine Normandin; Lama Talje; Hugo Lavoie; Guillaume Lépine; Sébastien Carréno; Benjamin H. Kwok; Gilles R. Hickson; Vincent Archambault
• Keywords: Polo-like kinase; Aurora B kinase; Aurora inhibitor; PLK1; Protein kinase domain; Midbody

Drosophila melanogaster Polo and its human orthologue Polo-like kinase 1 fulfill essential roles during cell division. Members of the (Plk) family contain an N-terminal domain (KD) a C-terminal Polo-Box (PBD), which mediates protein interactions. How Plks are regulated in cytokinesis is poorly understood. Here we show that phosphorylation by Aurora B required for cytokinesis. This activation loop KD promotes dissociation from PBD-bound microtubule-associated Map205, acts as allosteric inhibitor activity. mechanism allows release active microtubules central spindle recruitment to site Failure results both early late defects. Importantly, antagonistic regulation Map205 reveals interdomain mechanisms can play important controlling cellular functions Plks.