Active and inactive β1 integrins segregate into distinct nanoclusters in focal adhesions

• DOI: 10.1083/jcb.201707075
• Publication Date: 2018-04-09T14:10:18Z
• Authors: Matthias Spiess; Pablo Hernandez-V...; Anna Oddone; Helene Olofsson; Hans Blom; Dominic Waithe; John G. Lock; Melike Lakadamyali; Staffan Strömblad
• Keywords: Nanoclusters; STED microscopy

Integrins are the core constituents of cell-matrix adhesion complexes such as focal adhesions (FAs) and play key roles in physiology disease. fluctuate between active inactive conformations, yet whether activity state influences spatial organization integrins within FAs has remained unclear. In this study, we address question also ask integrin may be regulated either independently for each molecule or through locally coordinated mechanisms. We used two distinct superresolution microscopy techniques, stochastic optical reconstruction (STORM) stimulated emission depletion (STED), to visualize versus β1 integrins. first reveal a hierarchy with molecules arranged nanoclusters, which align form linear substructures that turn build FAs. Remarkably, FAs, segregate into nanoclusters being more organized. This unexpected segregation indicates synchronization activities implying existence coordinate mechanism regulation.