RENAL TRANSPLANTATION IN THE INBRED RAT

• DOI: 10.1084/jem.126.6.1099
• Publication Date: 2004-06-23T23:23:31Z
• Authors: R. D. Guttmann; C. B. Carpenter; R. R. Lindquist; J. P. Merrill
• Keywords: Heterologous; Allotransplantation; Thymocyte; Nephritis

Heterologous rabbit anti-rat thymocyte sera, its immunoglobulin G fraction, and the bivalent univalent antibody fragments obtained by pepsin digestion are potent immunosuppressive reagents when tested in a system of renal allotransplantation between LBN F1 hybrid Lewis rat strains. The AT F(ab')2 is not lymphocytotoxic vitro but has agglutinating ability, while Fab' neither agglutinates nor cytotoxic to lymphocytes, will inhibit reaction. IgG more immunogenic their host than normal F(ab')2, probably due increased delivery immune system. Donor pretreatment studies demonstrate that cross-reacting, highly with anti-lymphocyte specificity may bind sites be transferred new after transplantation. In addition, crude unabsorbed anti-thymocyte antisera induce nephritis characteristic complex disease which can eliminated complete absorption serum proteins. Further vivo evidence presented contains small amounts glomerular basement membrane antigens an autologous phase-nephrotoxic nephritis. amount binding GBM was reduced subcutaneous rather intravenous administration. Most contain low titer sheep erythrocytes experiments indicate nature immunodepressive effect globulin part passive transfer necessarily specific effect.