Shared idiotypic determinants on B and T lymphocytes reactive against the same antigenic determinants. II. Determination of frequency and characteristics of idiotypic T and B lymphocytes in normal rats using direct visualization.
Male
0301 basic medicine
B-Lymphocytes
Cell Survival
T-Lymphocytes
Receptors, Antigen, B-Cell
Cell Count
Rats, Inbred Strains
Antibodies, Anti-Idiotypic
Rats
Iodine Radioisotopes
Epitopes
03 medical and health sciences
Animals
Autoradiography
Female
Binding Sites, Antibody
Lymph Nodes
Spleen
Antilymphocyte Serum
DOI:
10.1084/jem.142.5.1218
Publication Date:
2004-06-23T23:23:31Z
AUTHORS (2)
ABSTRACT
Anti-idiotypic antibodies made against the antigen-binding receptors of T lymphocytes for a given antigen (Ag-B locus antigens in rats) can be shown to react with IgG same reactivity. Using such anti-idiotypic antibodies, normal Lewis B and type visualized by use anti-(Lewis-anti-DA) antibodies. Visualization was possible direct fluorescent antibody tests or autoradiography. first technique naked eye observations 6.2% expressed idiotypic markers signifying anti-DA reactivity, whereas anti-DA-reactive as measured this approach order 1.1%. Autoradiography purified gave similar figures. When comparing intensity fluorescence at single cell level using quantitative cytofluorometry reactive degree obtained anti-Ig lymphocytes.
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