Shared idiotypic determinants on B and T lymphocytes reactive against the same antigenic determinants. II. Determination of frequency and characteristics of idiotypic T and B lymphocytes in normal rats using direct visualization.

Male 0301 basic medicine B-Lymphocytes Cell Survival T-Lymphocytes Receptors, Antigen, B-Cell Cell Count Rats, Inbred Strains Antibodies, Anti-Idiotypic Rats Iodine Radioisotopes Epitopes 03 medical and health sciences Animals Autoradiography Female Binding Sites, Antibody Lymph Nodes Spleen Antilymphocyte Serum
DOI: 10.1084/jem.142.5.1218 Publication Date: 2004-06-23T23:23:31Z
ABSTRACT
Anti-idiotypic antibodies made against the antigen-binding receptors of T lymphocytes for a given antigen (Ag-B locus antigens in rats) can be shown to react with IgG same reactivity. Using such anti-idiotypic antibodies, normal Lewis B and type visualized by use anti-(Lewis-anti-DA) antibodies. Visualization was possible direct fluorescent antibody tests or autoradiography. first technique naked eye observations 6.2% expressed idiotypic markers signifying anti-DA reactivity, whereas anti-DA-reactive as measured this approach order 1.1%. Autoradiography purified gave similar figures. When comparing intensity fluorescence at single cell level using quantitative cytofluorometry reactive degree obtained anti-Ig lymphocytes.
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