Shared idiotypic determinants on B and T lymphocytes reactive against the same antigenic determinants. II. Determination of frequency and characteristics of idiotypic T and B lymphocytes in normal rats using direct visualization.

• DOI: 10.1084/jem.142.5.1218
• Publication Date: 2004-06-23T23:23:31Z
• Authors: H Binz; H Wigzell
• Keywords: Male; 0301 basic medicine; B-Lymphocytes; 0303 health sciences; Cell Survival; T-Lymphocytes; Receptors, Antigen, B-Cell; Cell Count; Rats, Inbred Strains; Antibodies, Anti-Idiotypic; Rats; Iodine Radioisotopes; Epitopes; 03 medical and health sciences; Animals; Autoradiography; Female; Binding Sites, Antibody; Lymph Nodes; Spleen; Antilymphocyte Serum

Anti-idiotypic antibodies made against the antigen-binding receptors of T lymphocytes for a given antigen (Ag-B locus antigens in rats) can be shown to react with IgG same reactivity. Using such anti-idiotypic antibodies, normal Lewis B and type visualized by use anti-(Lewis-anti-DA) antibodies. Visualization was possible direct fluorescent antibody tests or autoradiography. first technique naked eye observations 6.2% expressed idiotypic markers signifying anti-DA reactivity, whereas anti-DA-reactive as measured this approach order 1.1%. Autoradiography purified gave similar figures. When comparing intensity fluorescence at single cell level using quantitative cytofluorometry reactive degree obtained anti-Ig lymphocytes.