Clonotypic structures involved in antigen-specific human T cell function. Relationship to the T3 molecular complex.
clone (Java method)
DOI:
10.1084/jem.157.2.705
Publication Date:
2004-06-23T21:00:50Z
AUTHORS (6)
ABSTRACT
Monoclonal antibodies were produced against a human cytotoxic T cell clone, CT8III (specificity: HLA-A3), with the view of defining clonally restricted (clonotypic) surface molecules involved in its antigen recognition function. Two individual antibodies, termed anti-Ti1A and anti-Ti1B, reacted exclusively clone when tested on panel 80 additional clones from same donor, resting or activated cells, B macrophages, thymocytes, other hematopoietic cells. More importantly, two inhibited cell-mediated killing antigen-specific proliferation but did not affect functions any tested. This inhibition was secondary to generalized abrogation clone's function, because interleukin 2 responsiveness enhanced. To examine relationship structures defined by anti-clonotypic known molecules, antibody-induced modulation studies competitive binding assays performed. The results indicated that clonotypic associated with, distinct from, 20,000-mol wt T3 molecule expressed all mature lymphocytes. Moreover, contrast anti-T3, anti-Ti1B each immunoprecipitated 49,000 43,000-mol 131I-labeled cells under reducing conditions. development monoclonal such polymorphic should provide important probes further define receptor for antigen.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (0)
CITATIONS (594)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....