Clonotypic structures involved in antigen-specific human T cell function. Relationship to the T3 molecular complex.

• DOI: 10.1084/jem.157.2.705
• Publication Date: 2004-06-23T21:00:50Z
• Authors: S C Meuer; K A Fitzgerald; R E Hussey; J C Hodgdon; S F Schlossman; E L Reinherz
• Keywords: clone (Java method)

Monoclonal antibodies were produced against a human cytotoxic T cell clone, CT8III (specificity: HLA-A3), with the view of defining clonally restricted (clonotypic) surface molecules involved in its antigen recognition function. Two individual antibodies, termed anti-Ti1A and anti-Ti1B, reacted exclusively clone when tested on panel 80 additional clones from same donor, resting or activated cells, B macrophages, thymocytes, other hematopoietic cells. More importantly, two inhibited cell-mediated killing antigen-specific proliferation but did not affect functions any tested. This inhibition was secondary to generalized abrogation clone's function, because interleukin 2 responsiveness enhanced. To examine relationship structures defined by anti-clonotypic known molecules, antibody-induced modulation studies competitive binding assays performed. The results indicated that clonotypic associated with, distinct from, 20,000-mol wt T3 molecule expressed all mature lymphocytes. Moreover, contrast anti-T3, anti-Ti1B each immunoprecipitated 49,000 43,000-mol 131I-labeled cells under reducing conditions. development monoclonal such polymorphic should provide important probes further define receptor for antigen.