IgE class-restricted tolerance induced by neonatal administration of soluble or cell-bound IgE.

• DOI: 10.1084/jem.157.2.772
• Publication Date: 2004-06-23T21:00:50Z
• Authors: S S Chen; D H Katz
• Keywords: Splenocyte; Keyhole limpet hemocyanin; Hemocyanin; Polyclonal antibodies

Induction of IgE class-restricted tolerance was studied in high IgE-responder (BALB/c X SJL)F1 mice, which the parental BALB/c and SJL mice are low respectively. 2,4-Dinitrophenyl (DNP)-specific monoclonal administered to neonatally two forms: soluble at 250 micrograms per injection, or 10-100 ng coupled 25-50 10(6) syngeneic splenocytes by binding chemically reactive hapten trinitrobenzene sulfonate (TNBS) directly conjugated via a heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). Polyclonal induction observed treated with IgE-conjugated splenocytes. Thus these failed mount antibody responses either keyhole limpet hemocyanin ovalbumin challenge, assessed passive cutaneous anaphylaxis reaction. The IgG same antigens, however, were not affected this treatment. induced both forms persisted up least 6 mo repeated antigenic challenges. cells TNBS SPDP method prolonged 9 mo. polyclonal achieved only IgE, whereas DNP-specific IgG1 plus IgG2b modulate responses. In contrast, A/J)F1 strains responders, developed upon neonatal injection but splenocytes, indicating that gene products contributed effective presentation and/or recognition epsilon heavy chain molecules. Taken collectively, results demonstrated non-antigen-specific, isotype-restricted can be system. differential different tolerogen perhaps distinguishes underlying cellular mechanisms for tolerance.