Rescue of Daudi cell HLA expression by transfection of the mouse beta 2-microglobulin gene.

• DOI: 10.1084/jem.167.2.288
• Publication Date: 2004-06-24T07:56:10Z
• Authors: R H Seong; C A Clayberger; A M Krensky; J R Parnes
• Keywords: Beta-2 microglobulin; BETA (programming language); CTL*

The Daudi cell line is a B-lymphoblastoid derived from Burkitt lymphoma. cells lack surface expression of class I HLA molecules despite the presence intracellular heavy chains. They have defect in gene encoding beta 2-microglobulin (beta 2m), resulting translatable mRNA for this protein. It has been thought that deficiency responsible expression. However, data recently presented demonstrating at least one mouse chain can be expressed on absence 2m. These results raised questions whether 2m only and transfer single could restore We found transfection into indeed rescued molecules, these recognized both by monomorphic allospecific mAbs. CTL clones specific HLA-B17 or determinant shared HLA-A2 killed transfected with gene, but not untransfected vector alone. Mouse exchange human when were incubated serum. This did alter ability to CTLs. demonstrate sole reason cells, required chains Daudi.