PECAM-1 is required for transendothelial migration of leukocytes.

• DOI: 10.1084/jem.178.2.449
• Publication Date: 2004-06-24T07:56:10Z
• Authors: W A Muller; S A Weigl; X Deng; D M Phillips
• Keywords: CD31; Cell–cell interaction; Monocyte

Platelet/endothelial cell adhesion molecule 1 (PECAM-1; CD31) is crucial to the process of leukocyte transmigration through intercellular junctions vascular endothelial cells. A monoclonal antibody PECAM, or recombinant soluble blocks transendothelial migration monocytes by 70-90%. Pretreating either with transmigration. If endothelium first activated cytokines, anti-PECAM PECAM again block both and neutrophils. Anti-PECAM does not chemotaxis type. Light electron microscopy reveal that leukocytes blocked in remain tightly bound apical surface cell, precisely over junction. Thus, emigration can be dissected into three successive stages: rolling, mediated selectin class molecules; tight adhesion, integrins their counter-receptors; now transmigration, which, based on these studies, requires PECAM-1.