Melanocyte lineage-specific antigen gp100 is recognized by melanoma-derived tumor-infiltrating lymphocytes.

• DOI: 10.1084/jem.179.3.1005
• Publication Date: 2004-06-24T07:56:10Z
• Authors: A B Bakker; M W Schreurs; A J de Boer; Y Kawakami; S A Rosenberg; G J Adema; C G Figdor
• Keywords: CTL*; clone (Java method); Tumor-infiltrating lymphocytes

We recently isolated a cDNA clone that encodes the melanocyte lineage-specific antigen glycoprotein (gp)100. Antibodies directed against gp100 are an important tool in diagnosis of human melanoma. Since is highly expressed melanocytic cells, we investigated whether this might serve as target for antimelanoma cytotoxic T lymphocytes (CTL). Here, demonstrate tumor-infiltrating (TIL) derived from melanoma patient (TIL 1200) gp100. HLA-A2.1+ cells lysed by TIL patient. In addition, murine double transfectants, expressing both HLA-A2.1 and gp100, 1200, whereas transfectants only not susceptible to lysis. Furthermore, cell line BLM, which lacks expression resistant lysis, becomes after transfection cDNA. Finally, normal melanocytes 1200. These data differentiation can be recognized context CTL Gp100 may therefore constitute useful specific immunotherapy melanoma, provided no unacceptable cytotoxicity towards tissue observed.