Interferon gamma upregulates its own gene expression in mouse peritoneal macrophages.

• DOI: 10.1084/jem.179.5.1731
• Publication Date: 2004-06-24T07:56:10Z
• Authors: P Di Marzio; P Puddu; L Conti; F Belardelli; S Gessani
• Keywords: Male; 0301 basic medicine; Mice, Inbred C3H; Base Sequence; Molecular Sequence Data; Mice, Nude; DNA; Polymerase Chain Reaction; Up-Regulation; 3. Good health; Interferon-gamma; Mice; 03 medical and health sciences; Gene Expression Regulation; Macrophages, Peritoneal; Animals; Cells, Cultured

Interferon gamma (IFN-gamma) exerts a variety of immunoregulatory effects on several cell targets. It is generally assumed that IFN-gamma specifically produced by T and large granular lymphocytes. In this study, we show constitutively expressed in resting mouse peritoneal macrophages (PM). Treatment PM with cycloheximide results significant accumulation mRNA, suggesting short-lived mRNA accumulates when protein synthesis inhibited. Moreover, treatment also clear-cut mRNA. This effect not observed murine lymphocytes from mesenteric lymph nodes (which instead produce after phytohemagglutinin treatment) lines. The secretion 24-48 h. upregulation expression found anti-asialo GM1-treated nude mice. We suggest the ability to indicative an autocrine mechanism. macrophage may play role regulation differentiation immune response.