gp39-CD40 interactions are essential for germinal center formation and the development of B cell memory.

• DOI: 10.1084/jem.180.1.157
• Publication Date: 2004-06-24T07:56:10Z
• Authors: T M Foy; J D Laman; J A Ledbetter; A Aruffo; E Claassen; R J Noelle
• Keywords: Memory B cell

gp39, the ligand for CD40 expressed on activated CD4+ T helper cells, is required generation of antibody responses to T-dependent (TD) antigens. Treatment mice with anti-gp39 in vivo inhibits both primary and secondary formation TD, but not T-independent However, role this receptor-ligand pair development germinal centers B cell memory as yet undefined. Using an study examines requirement gp39-CD40 interactions induction center formation, well memory. Animals were immunized, treated anti-gp39, evaluated using immunohistochemical staining presence splenic 9-11 d after immunization. The results demonstrate that was completely inhibited a result treatment anti-gp39. Moreover, adoptive transfer experiments antigen-specific cells also consequence blocking interactions. Taken together, data are critical only responses,