Interleukin-10 Is a Natural Suppressor of Cytokine Production and Inflammation in a Murine Model of Allergic Bronchopulmonary Aspergillosis

• DOI: 10.1084/jem.185.6.1089
• Publication Date: 2002-07-26T16:49:30Z
• Authors: Gabriele Grünig; David B. Corry; Michael W. Leach; Brian W.P. Seymour; Viswanath P. Kurup; Donna M. Rennick
• Keywords: Allergic bronchopulmonary aspergillosis; Priming (agriculture)

We have used interleukin-10 (IL-10) gene knockout mice (IL-10−/−) to examine the role of endogenous IL-10 in allergic lung responses Aspergillus fumigatus Ag. In vitro restimulated cells from sensitized IL-10−/− produced exaggerated amounts IL-4, IL-5, and interferon-γ (IFN-γ) compared with wild-type (WT) cells. vivo, significance regulating repeated A. inhalation was strikingly revealed outbred that had a 50–60% mortality rate, while rare similarly treated WT mice. Furthermore, exhibited airway inflammation heightened levels IL-5 IFN-γ bronchoalveolar lavage (BAL) fluids. contrast, magnitude response similar intranasally (i.n.) different strain (C57BL/6). Using route priming (intraperitoneal) followed by one i.n. challenge we found C57BL/6 eosinophilic inflammation, BAL–IL-5 levels, numbers αβT tissues conclude can suppress inflammatory Th2-like as well Th1-like given constraints genetic background priming.