The Human C3a Receptor Is Expressed on Neutrophils and Monocytes, but Not on B or T Lymphocytes

• DOI: 10.1084/jem.186.2.199
• Publication Date: 2002-07-26T16:49:30Z
• Authors: Ulrich Martin; Daniel Bock; Lubomir Arseniev; Mark A. Tornetta; Robert S. Ames; Wilfried Bautsch; Jörg Köhl; Arnold Ganser; Andreas Klos
• Keywords: Anaphylatoxin

The pathophysiological relevance of the complement split product C3a as a proinflammatory mediator is still ill defined. expression pattern human receptor (C3aR) can provide important clues for role this anaphylatoxin in inflammation. There strong evidence C3aR on basophils, and eosinophils, but additionally, only tumor cell lines leukemic or hepatic origin. It unclear whether neutrophils also express C3aR, need costimulus provided by eosinophils certain biological responses, lack respond to via secondary stimulus generated i.e., an indirect mode. In present study, polyclonal antiserum raised against second extracellular loop was used characterize peripheral blood leukocytes. For high degree purification neutrophils, negative selection method established that decreased contamination with CD9bright+ down <0.2%. Flow cytometric analyses, functional assays, binding assays highly purified confirmed coupling. Monocytes were identified additional C3aR-positive population blood. could be confirmed. contrast, not detected unchallenged B T lymphocytes (or lymphocyte-derived Raji cells).