The Human C3a Receptor Is Expressed on Neutrophils and Monocytes, but Not on B or T Lymphocytes

0301 basic medicine B-Lymphocytes Membrane Glycoproteins Neutrophils T-Lymphocytes Blotting, Northern Flow Cytometry Monocytes Tetraspanin 29 Rats Receptors, Complement 3. Good health 03 medical and health sciences 0302 clinical medicine Antigens, CD Complement C3a Animals Humans Calcium RNA, Messenger Rabbits
DOI: 10.1084/jem.186.2.199 Publication Date: 2002-07-26T16:49:30Z
ABSTRACT
The pathophysiological relevance of the complement split product C3a as a proinflammatory mediator is still ill defined. The expression pattern of the human C3a receptor (C3aR) can provide important clues for the role of this anaphylatoxin in inflammation. There is strong evidence for C3aR expression on basophils, and eosinophils, but additionally, only on tumor cell lines of leukemic or hepatic origin. It is unclear whether neutrophils also express the C3aR, but need a costimulus provided by eosinophils for certain biological responses, or whether neutrophils lack the C3aR and respond to C3a via a secondary stimulus generated by eosinophils, i.e., by an indirect mode. In the present study, polyclonal antiserum raised against the second extracellular loop of the C3aR was used to characterize C3aR expression on peripheral blood leukocytes. For high degree purification of neutrophils, a negative selection method was established that decreased the contamination with CD9bright+ eosinophils down to <0.2%. Flow cytometric analyses, functional assays, and binding assays on highly purified neutrophils confirmed C3aR expression and coupling. Monocytes were identified as an additional C3aR-positive cell population of the peripheral blood. The expression of the C3aR on eosinophils could be confirmed. In contrast, the receptor could not be detected on unchallenged B or T lymphocytes (or lymphocyte-derived Raji cells).
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