The α Chemokine, Interleukin 8, Inhibits the Antiviral Action of Interferon α

• DOI: 10.1084/jem.186.7.1077
• Publication Date: 2002-07-26T16:49:30Z
• Authors: Khalid S.A. Khabar; Fahad Al-Zoghaibi; Mohammed N. Al-Ahdal; Tsugiya Murayama; Mohammed Dhalla; Naofumi Mukaida; Mohammed Taha; Sultan T. Al-Sedairy; Yunus Siddiqui; George Kessie; Kouji Matsushima
• Keywords: Gene Expression Regulation, Viral; 0301 basic medicine; Dose-Response Relationship, Drug; Cell Survival; Interleukin-8; Antibodies, Monoclonal; Interferon-alpha; Picornaviridae; Receptors, Interleukin; Antiviral Agents; Binding, Competitive; Recombinant Proteins; Cell Line; Receptors, Interleukin-8A; 3. Good health; 03 medical and health sciences; Cytopathogenic Effect, Viral; Antigens, CD; Chlorocebus aethiops; 2',5'-Oligoadenylate Synthetase; Animals; Humans; RNA, Messenger

Interferon (IFN) exhibits a potent antiviral activity in vitro and plays major role the early defense against viruses. Like IFN, proinflammatory chemokine, interleukin (IL)-8, is induced by viruses appears circulation during viral infections. In an cytopathic effect assay for we found that IL-8 can inhibit IFN-alpha dose-dependent manner. This action was reversed specific monoclonal antibodies to IL-8. The chemokine able attenuate IFN-mediated inhibition of replication as determined measuring infectious virus yield. also diminished ability IFN stage since attenuated formation proteins. It appeared interfered with late rather than step pathway such gene expression. inhibitory on associated reduced 2',5'-A oligoadenylate synthetase activity, well correlative anti- encephalomyocarditis IFN-alpha. Understanding pathways antagonize may lead novel approaches potentiate endogenous therapeutic IFN.