A Natural Immunological Adjuvant Enhances T Cell Clonal Expansion through a CD28-dependent, Interleukin (IL)-2–independent Mechanism

• DOI: 10.1084/jem.187.2.225
• Publication Date: 2002-07-26T16:49:30Z
• Authors: Alexander Khoruts; Anna Mondino; Kathryn A. Pape; Steven L. Reiner; Marc K. Jenkins
• Keywords: Adoptive Cell Transfer

The adoptive transfer of naive CD4+ T cell receptor (TCR) transgenic cells was used to investigate the mechanisms by which adjuvant lipopolysaccharide (LPS) enhance clonal expansion in vivo. Subcutaneous administration soluble antigen (Ag) resulted rapid and transient accumulation Ag-specific draining lymph nodes (LNs), preceded production interleukin (IL)-2. CD28-deficient, produced only small amounts IL-2 response Ag did not accumulate LN same extent as wild-type cells. Injection LPS, a natural immunological adjuvant, enhanced wild-type, but had no significant effect on Therefore, CD28 is critical for Ag-driven proliferation vivo, essential LPS-mediated enhancement these events. However, could explain LPS-dependent increase because IL-2–deficient, accumulated greater than plus LPS. These results indicate that adjuvants improve vivo via CD28-dependent signal can operate absence IL-2.