An Essential Role for Interleukin 10 in the Function of Regulatory T Cells That Inhibit Intestinal Inflammation

• DOI: 10.1084/jem.190.7.995
• Publication Date: 2002-07-26T16:48:33Z
• Authors: Chrystelle Asseman; Smita Mauze; Michael W. Leach; Robert L. Coffman; Fiona Powrie
• Keywords: CD4-Positive T-Lymphocytes; Inflammation; Mice, Knockout; 0301 basic medicine; Mice, Inbred BALB C; Colon; Antibodies, Monoclonal; Mice, SCID; Th1 Cells; Interleukin-10; 3. Good health; DNA-Binding Proteins; Colonic Diseases; Interferon-gamma; Mice; 03 medical and health sciences; 0302 clinical medicine; Transforming Growth Factor beta; Animals; Intestinal Mucosa; Immunity, Mucosal; Spleen

A T helper cell type 1–mediated colitis develops in severe combined immunodeficient mice after transfer of CD45RBhigh CD4+ cells and can be prevented by cotransfer the CD45RBlow subset. The immune-suppressive activities population reversed vivo administration an anti-transforming growth factor β antibody. Here we show that interleukin (IL)-10 is essential mediator regulatory functions population. This isolated from IL-10–deficient (IL-10−/−) was unable to protect when transferred alone immune-deficient recipients induced colitis. Treatment with anti–murine IL-10 receptor monoclonal antibody abrogated inhibition mediated wild-type (WT) cells, suggesting necessary for effector function Inhibition WT not dependent on production progeny as were able inhibit IL-10−/− cells. These findings provide first clear evidence plays a nonredundant role functioning control inflammatory responses towards intestinal antigens.