Antimicrobial Actions of the Nadph Phagocyte Oxidase and Inducible Nitric Oxide Synthase in Experimental Salmonellosis. II. Effects on Microbial Proliferation and Host Survival in Vivo

• DOI: 10.1084/jem.192.2.237
• Publication Date: 2002-07-26T16:48:33Z
• Authors: Pietro Mastroeni; Andrés Vazquez-To...; Ferric C. Fang; Yisheng Xu; Shahid Khan; Carlos E. Hormaeche; Gordon Dougan
• Keywords: Phagocyte; Congenic; Mononuclear phagocyte system

The roles of the NADPH phagocyte oxidase (phox) and inducible nitric oxide synthase (iNOS) in host resistance to virulent Salmonella typhimurium were investigated gp91phox−/−, iNOS−/−, congenic wild-type mice. Although both gp91phox−/− iNOS−/− mice demonstrated increased susceptibility infection with S. compared mice, kinetics bacterial replication dramatically different mouse strains. Greater numbers present spleens livers C57BL/6 controls as early day 1 infection, all succumbed within 5 d. In contrast, an burden was detected reticuloendothelial organs only beyond first week infection. Influx inflammatory CD11b+ cells, granuloma formation, serum interferon γ levels unimpaired but iNOS-deficient granulomas unable limit replication. phagocye iNOS are required for Salmonella, appear operate principally at stages