Integrin αMβ2-Mediated Cell Migration to Fibrinogen and Its Recognition Peptides

• DOI: 10.1084/jem.193.10.1123
• Publication Date: 2002-07-26T16:48:33Z
• Authors: Christopher B. Fo...; Dmitry A. Solovjov; Tatiana P. Ugarova; Edward F. Plow
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; 3. Good health

Leukocyte migration is the hallmark of inflammation, and integrin αMβ2 its ligand fibrinogen (Fg) are key participants in this cellular response. Cells expressing wild-type or mutant Fg derivatives have been used to dissect molecular requirements for receptor–ligand pair mediate cell migration. The major conclusions that (a) Fg, D fragment, P1 P2 recognition peptides support a chemotactic response; (b) when I domain αL was replaced with αM, chimeric receptor supported Fg; however, αM subunit, containing but lacking β2 poorly, thus, αMI necessary not sufficient chemotaxis, efficient requires subunit domain; (c) addition supporting migration, enhanced αMβ2-mediated chemotaxis peptide. This activation associated exposure activation-dependent epitope recognized by monoclonal antibody 7E3 observed also human neutrophils. Taken together, these data define specific assign novel proinflammatory activity