Mutant p53 facilitates somatic cell reprogramming and augments the malignant potential of reprogrammed cells

• DOI: 10.1084/jem.20100797
• Publication Date: 2010-08-10T02:59:02Z
• Authors: Rachel Sarig; Noa Rivlin; Ran Brosh; Chamutal Bornstein; Iris Kamer; Osnat Ezra; Alina Molchadsky; Naomi Goldfinger; Ori Brenner; Varda Rotter
• Keywords: Reprogramming; KLF4

p53 deficiency enhances the efficiency of somatic cell reprogramming to a pluripotent state. As is usually mutated in human tumors and many forms gain novel activities, we studied influence mutant (mut-p53) on reprogramming. Our data indicate function (GOF) property for mut-p53, which markedly enhanced process compared with deficiency. Importantly, this activity mut-p53 induced alterations characteristics reprogrammed cells. Although knockout (KO) cells only Oct4 Sox2 maintained their capacity vivo, expressing lost capability gave rise malignant tumors. This GOF not attributed its effect proliferation, as both KO displayed similar proliferation rates. In addition, demonstrate an oncogenic Klf4, overexpression either or aggressive Overall, our show that differentiate into three germ layers vitro can form tumors, suggesting genetically unstable cells, such those mutated, may result generation tumor-forming potential.