Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow

• DOI: 10.1084/jem.20141442
• Publication Date: 2015-02-17T03:07:11Z
• Authors: Jaeyop Lee; Gaëlle Breton; Thiago Yukio Kiku...; Yu Jerry Zhou; Arafat Aljoufi; Sarah Puhr; Mark J. Cameron; Rafick-Pierre Sékaly; Michel C. Nussenz...; Kang Liu
• Keywords: Monocyte; Cord blood; Progenitor

In mice, two restricted dendritic cell (DC) progenitors, macrophage/dendritic progenitors (MDPs) and common (CDPs), demonstrate increasing commitment to the DC lineage, as they sequentially lose granulocyte monocyte potential, respectively. Identifying these has enabled us understand role of DCs monocytes in immunity tolerance mice. humans, however, remain unknown. Progress studying human development been hampered by lack an vitro culture system that recapitulates vivo hematopoiesis. Here we report a supports CD34+ hematopoietic stem into three major subsets, monocytes, granulocytes, NK B cells. Using this system, defined pathway for revealed sequential origin from increasingly progenitors: granulocyte-monocyte-DC progenitor (hGMDP) develops monocyte-dendritic (hMDP), which turn CDP (hCDP) is produce subsets. The phenotype partially overlaps with granulocyte-macrophage (GMPs). These reside cord blood bone marrow but not or lymphoid tissues.