Dissecting hematopoietic and renal cell heterogeneity in adult zebrafish at single-cell resolution using RNA sequencing

• DOI: 10.1084/jem.20170976
• Publication Date: 2017-09-06T21:25:14Z
• Authors: Qin Tang; Sowmya Iyer; Riadh Lobbardi; John C. Moore; Huidong Chen; Caleb Lareau; Christine Hebert; McKenzie L. Shaw; Cyril Neftel; Mario L. Suva; Craig J. Ceol; Andre Bernards; Martin Aryee; Luca Pinello; Iain A. Drummond; David M. Langenau
• Keywords: Single-Cell Analysis

Recent advances in single-cell, transcriptomic profiling have provided unprecedented access to investigate cell heterogeneity during tissue and organ development. In this study, we used massively parallel, single-cell RNA sequencing define within the zebrafish kidney marrow, constructing a comprehensive molecular atlas of definitive hematopoiesis functionally distinct renal cells found adult zebrafish. Because our method analyzed blood an unbiased manner, approach was useful characterizing immune-cell deficiencies DNA-protein kinase catalytic subunit (prkdc), interleukin-2 receptor γ (il2rga), double-homozygous-mutant fish, identifying losses T, B, natural killer specific genetic mutants. Our analysis also uncovered novel types, including two classes immune cells, classically defined erythroid-primed hematopoietic stem progenitor mucin-secreting stem/progenitor cells. total, work provides first, comprehensive, marrow