Endothelial cell fitness dictates the source of regenerating liver vasculature

• DOI: 10.1084/jem.20180008
• Publication Date: 2018-09-07T15:45:43Z
• Authors: Mahak Singhal; Xiaoting Liu; Donato Inverso; Kai Jiang; Jianing Dai; Hao He; Susanne Bartels; Weiping Li; Ashik Ahmed Abdul...; Nicolas Gengenbacher; Eva Besemfelder; Lijian Hui; Hellmut G. Augustin; Junhao Hu
• Keywords: Liver Regeneration

Neoangiogenesis plays a key role in diverse pathophysiological conditions, including liver regeneration. Yet, the source of new endothelial cells (ECs) remains elusive. By analyzing regeneration vasculature irradiation-based myeloablative and nonmyeloablative bone marrow transplantation mouse models, we discovered that neoangiogenesis livers with intact endothelium was solely mediated by proliferation resident ECs. However, following irradiation-induced EC damage, marrow–derived mononuclear were recruited incorporated into vasculature. Further experiments direct infusion or granulocyte colony–stimulating factor (G-CSF)–mediated progenitor cell mobilization, which resembles clinically relevant stem therapy, demonstrated did not contribute to after two-thirds partial hepatectomy (PHx). Taken together, data reconcile many discrepancies literature highlight cellular regenerating depends on fitness residual