The lifespan and kinetics of human dendritic cell subsets and their precursors in health and inflammation

• DOI: 10.1084/jem.20220867
• Publication Date: 2024-10-17T14:07:41Z
• Authors: Ruth Lubin; Amit A. Patel; Jonas Mackerodt; Yan Zhang; Rotem Gvili; Kevin Mulder; Charles-Antoine D...; Parinaaz Jalali; James R.W. Glanville; Idit Hazan; Nikhila Sridharan; Gurion Rivkin; Ayse Akarca; Teresa Marafioti; Derek W. Gilroy; Leonid Kandel; Alexander Mildner; Asaf Wilensky; Becca Asquith; Florent Ginhoux; Derek Macallan; Simon Yona
• Keywords: Inflammation; Male; Adult; 570; Kinetics; 610; Humans; Cell Differentiation; Female; Dendritic Cells; Axl Receptor Tyrosine Kinase

Dendritic cells (DC) are specialized mononuclear phagocytes that link innate and adaptive immunity. They comprise two principal subsets: plasmacytoid DC (pDC) conventional (cDC). Understanding the generation, differentiation, migration of cDC is critical for immune homeostasis. Through human in vivo deuterium-glucose labeling, we observed rapid appearance AXL+ Siglec6+ (ASDC) bloodstream. ASDC circulate ∼2.16 days, while cDC1 DC2 ∼1.32 ∼2.20 respectively, upon release from bone marrow. Interestingly, DC3, a subset shares several similarities with monocytes, exhibits labeling profile closely resembling DC2. In model cutaneous inflammation, were recruited to inflammatory site, displaying distinctive effector signature. Taken together, these results quantify ephemeral circulating lifespan propose functions their precursors rapidly sites inflammation.