The Legs Have It: In Situ Expression of Ion Transporters V-Type H+-ATPase and Na+/K+-ATPase in the Osmoregulatory Leg Organs of the Invading CopepodEurytemora affinis

• DOI: 10.1086/686323
• Publication Date: 2016-04-14T18:53:33Z
• Authors: Lucie Gerber; Carol Eunmi Lee; Evelyse Grousset; Eva Blondeau-Bidet; Nesrine Boudour B...; Catherine Lorin-N...; Mireille Charmant...; Guy Charmantier
• Keywords: Male; 0301 basic medicine; Salinity; Extremities; Water-Electrolyte Balance; Gene Expression Regulation, Enzymologic; 6. Clean water; Copepoda; Proton-Translocating ATPases; 03 medical and health sciences; Osmoregulation; Animals; Female; Sodium-Potassium-Exchanging ATPase

The copepod Eurytemora affinis has an unusually broad salinity range, as some populations have recently invaded freshwater habitats independently from their ancestral saline habitats. Prior studies shown evolutionary shifts in ion transporter activity during invasions and localization of transporters newly discovered "Crusalis organs" the swimming legs. goals this study were to localize quantify expression transport enzymes V-type H(+)-ATPase (VHA) Na(+)/K(+)-ATPase (NKA) legs E. determine degree involvement each leg ionic regulation. We confirmed presence two distinct types ionocytes Crusalis organs. Both cell expressed VHA NKA, population location NKA was, respectively, apical basal. Quantification situ established predominance pairs 3 4 both populations. Increases (in fresh water) relative (at 15 PSU) arose increase abundance per rather than number ionocytes. This result suggests a simple mechanism for increasing uptake water. In contrast, decline decrease ionocyte area 4, likely resulting decreases or size containing NKA. Such results provide insights into mechanisms regulation species, with added underlying physiological adaptation habitat invasions.