Hemocompatibility of dextran-graft-polyacrylamide/zinc oxide nanosystems: hemolysis or eryptosis?

• DOI: 10.1088/1361-6528/ad02a3
• Publication Date: 2023-10-12T22:21:26Z
• Authors: Anatolii I Onishc...; Volodymyr Yu Prok...; Vasyl A Chumachenko; Pavlo A Virych; Liliya Y Tryfonyuk; Nataliya V Kutsevol; Anton S Tkachenko
• Keywords: Nanotoxicology; Erythrocyte fragility

Aim. In this study, blood compatibility of ZnO nanoparticles-polymer nanocomplex (D-PAA/ZnONPs(SO42-)) synthesizedin situinto dextran-graft-polyacrylamide (D-PAA) using zinc sulphate as a precursor was tested hemolysis, osmotic fragility and eryptosis assays.Materials methods. Dose-dependent ability to induce assessed following 24 h incubation at concentrations 0-800 mg l-1analyzing hallmarks (cell shrinkage phosphatidylserine externalization), well reactive oxygen species generation. Hemolysis detected spectrophotometrically based on hemoglobin release exposure the D-PAA/ZnONPs(SO42-) nanocomplex. Osmotic test (OFT) involved detection hemolysis red cells exposed 0.2% saline solution with Additional in presence or absence either ascorbic acid EGTA used reveal implication oxidative stress- Ca2+-mediated mechanisms nanocomplex-induced erythrotoxicity.Results. Hemocompatibility assessment revealed that it induced reduced resistance erythrocytes stress above 400 200 l-1, respectively. Oxidative were not hemolysis. Strikingly, did promote cell membrane scrambling, direct for h. Thus, eryptosisin vitro. Eryptosis is generally considered occur earlier than response order prevent hemolytic death. Counterintuitively, our data suggest can be triggered by nanomaterials prior indicating assays should combination testing nanomaterials.Conclusions. The has good hemocompatibility profile low concentrations. nanotoxicology include both assays.