A cell-laden hybrid fiber/hydrogel composite for ligament regeneration with improved cell delivery and infiltration

• DOI: 10.1088/1748-605x/aa7b51
• Publication Date: 2017-09-25T07:15:39Z
• Authors: Jeong Hwa Kim; Yeong-Jin Choi; Hee-Gyeong Yi; Joon Ho Wang; Dong-Woo Cho; Young Hun Jeong
• Keywords: 0301 basic medicine; Cell Survival; Mesenchymal Stem Cell Transplantation; CONSTRUCTS; nano-/microfibers; 03 medical and health sciences; ligament regeneration; Humans; Regeneration; RECONSTRUCTION; ELECTROSPUN SCAFFOLDS; Cell Proliferation; mesenchymal stem cells; Tissue Engineering; Tissue Scaffolds; HYDROGEL; Gene Expression Profiling; Temperature; Cell Differentiation; Hydrogels; Mesenchymal Stem Cells; IN-VITRO; DIFFERENTIATION; Phenotype; Intercellular Signaling Peptides and Proteins; Stress, Mechanical; TISSUE-ENGINEERING SCAFFOLDS; BONE; STEM-CELLS; ANTERIOR CRUCIATE LIGAMENT

Ligament, a fibrous connective tissue between bones, is a unique tissue in human anatomy because it has complex viscoelastic properties and is very tough. Moreover, it is an important tissue for regeneration because frequent injuries occur, but there are limited types of substitutes that can be used as a tissue replacement. In this study, we present a stem cell-laden fiber/hydrogel composite structure with a layered fibrous structure, which can enhance cell infiltration, topographical cue and mechanical properties. It can promote cell viability, proliferation, and differentiation of the ligament phenotype with the help of a growth factor. The mechanical properties of the developed structure were experimentally identified using tensile tests, while cell viability and various functionalities were verified through culture tests using mesenchymal stem cells.