Characterization of HIV Replication Complexes Early after Cell-to-Cell Infection

• DOI: 10.1089/aid.1993.9.817
• Publication Date: 2009-03-16T17:23:04Z
• Authors: LITSA KARAGEORGOS; PENG LI; CHRIS BURRELL
• Keywords: Replication factor C

In this study, we have characterized the HIV DNA-containing replication complexes present in cells early after cell-to-cell infection, using sucrose gradient sedimentation and immunoprecipitation. Six hours a cytoplasmic complex sedimented as large structure (320S). This was precipitated by antisera to three virus-coded enzymes (reverse transcriptase, integrase, protease), matrix protein (p17), cellular histones but not major capsid (p24). associated with cell membranes could be dissociated into smaller discrete subunits, detergents. Nuclear extracts from same infection contained (80S) that lacked reverse transcriptase (p17). Cytoplasmic cell-free virus 160S structures under identical conditions, previously reported. Our results indicate that, following transmission of HIV, all pol gene products, p17, are taking part or completed transcription. Transportation nucleus is structural changes, including reduction size altered composition.