HIV Proteins Regulate Bone Marker Secretion and Transcription Factor Activity in Cultured Human Osteoblasts with Consequent Potential Implications for Osteoblast Function and Development

• DOI: 10.1089/aid.2007.0112
• Publication Date: 2007-12-26T18:26:50Z
• Authors: Eoin J. Cotter; Andrea P. Malizia; Nicholas Chew; William G. Powderly; Peter P. Doran
• Keywords: Transcription

A high incidence of decreased bone mineral density (BMD) has increasingly been associated with HIV infection. In this study mesenchymal stem cell (MSC) and human osteoblast (hOB) lines were treated tat, rev, p55-gag, gp120 HTLV env (100 ng/ml, 24 h). Cells then analyzed for calcium deposition, alkaline phosphatase (ALP) activity, lipid levels using established methods. Real-time PCR gene-specific primers was used to quantify the mRNA transcription factors RUNX-2 PPARgamma, known be pro-osteogenic pro-adipogenic, respectively. The secreted markers factor activity determined commercial assays. OBs, p55-gag seen reduce ALP BMP-2, -7, RANK-L, expression RUNX-2. osteocalcin also significantly reduced by treatment, while increased PPARgamma activity. Lipid treatment. ability MSCs develop into functioning OBs affected presence proteins, inducing a decrease in osteogenesis, rev induced an increase. proteins can potentially modulate OB development function vitro via modulation maker secretion