HIV Type 1polGene Diversity and Antiretroviral Drug Resistance Mutations in Santos, Brazil

• DOI: 10.1089/aid.2007.0203
• Publication Date: 2008-03-07T14:44:49Z
• Authors: Dercy José de Sa-...; Marta da Silva So...; Valéria Candido; Luiz Henrique Gag...; Elizabeth Cavaliere; Ricardo Sobhie Diaz; Marcos Montani Ca...
• Keywords: Resistance mutation

HIV-1 antiretroviral drug resistance mutations in subtype B, F, and recombinants B/F Santos, Brazil were characterized. We studied 83 samples from individuals enrolled at the Brazilian HIV/AIDS programs Santos. These patients have been treated with multiantiretroviral therapy. Samples collected 2006; RNA was extracted plasma used as a target to amplify pol gene of HIV-1. PCR products sequenced on both strands, phylogenetic analyses performed by neighbor-joining, recombination evaluated bootscan. sequencing revealed that 54 strains belonged 4 1 C, 24 recombinants. Recombinant break points 20 are same identified CRF28_BF CRF29_BF. Drug common subtypes protease inhibitors M46I/L (29%), I54V (24%), A71V (22%), V82A/F (31%); reverse transcriptase nucleoside M41L (52%), D67N (30%), K70R (26%), M184V (88%), L210W T215Y/I/F (65%), K219Q/E/N (28%); nonnucleoside mutation K103N (52%). Our results suggest that, general, amino acids emerging recombinant forms BF due selective pressure antiretrovirals. CRF29_BF recognized important for evolution local epidemic.