Reciprocal Relationship Between Reactive Oxygen Species and Cyclooxygenase-2 and Vascular Dysfunction in Hypertension

• DOI: 10.1089/ars.2011.4335
• Publication Date: 2012-06-06T14:59:41Z
• Authors: Sonia Martínez-Re...; María S. Avendaño; Ana B. García-Red...; Yolanda Álvarez; Andrea Aguado; Jose V. Pérez-Girón; Laura García-Redondo; Vanesa Esteban; Juan M. Redondo; María J. Alonso; Ana M. Briones; Mercedes Salaices
• Keywords: Apocynin; NAD(P)H oxidase; SOD2; Phenylephrine

Aims: This study evaluates a possible relationship between reactive oxygen species (ROS) and cyclooxygenase (COX)-2-derived products in conductance resistance arteries from hypertensive animals. Angiotensin II (Ang II)-infused mice or spontaneously rats treated with the NAD(P)H Oxidase inhibitor apocynin, mitochondrion-targeted SOD2 mimetic Mito-TEMPO, superoxide dismutase analog tempol, COX-2 Celecoxib were used. Results: Apocynin, treatments prevented Ang II-induced hypertension, increased vasoconstrictor responses to phenylephrine, reduced acetylcholine relaxation. The NOX-2 gp91ds-tat, NOX-1 ML171, catalase, NS398 abolished ex vivo effect of II-enhancing phenylephrine responses. Antioxidant diminished vascular expression, prostanoid production, and/or participation COX-derived contractile prostanoids thromboxane A2 receptor (TP) responses, observed models. treatment normalized ROS production (O2·− H2O2), expression (NOX-1, NOX-4, p22phox) activity, MnSOD both Apocynin Mito-TEMPO also these parameters oxidative stress. improved nitric oxide (NO) modulation by NO model. Innovation: provides mechanistic evidence circuitous hypertension. Conclusion: excess mitochondria COX-2/TP axis act concert induce dysfunction Antioxid. Redox Signal. 18, 51–65.