The Long Noncoding RNA LINC00963 Inhibits Corneal Fibrosis Scar Formation by Targeting miR-143-3p

• DOI: 10.1089/dna.2021.1034
• Publication Date: 2022-03-09T16:25:05Z
• Authors: Lixia Zhang; Jinning Gao; Anjing Gong; Yanhan Dong; Xiaodan Hao; Xuekang Wang; Jian Zheng; Wenmeng Ma; Yiying Song; Jie Zhang; Wenhua Xu
• Keywords: Myofibroblast

Corneal fibrosis is a complication of severe corneal injury, one the major causes vision loss. The formation myofibroblasts has emerged as key stimulative factor fibrosis. In current study, we focused on role LINC00963 in regulating Transforming growth β1 (TGF-β1) was used to induce human stromal cells differentiating into myofibroblasts, and significant increase α-smooth muscle actin (α-SMA) verified by quantitative real-time PCR (qRT-PCR), western blot, immunofluorescence, respectively. identified be one-half decreased compared with nonstimulated cells, indicating that it might play Interestingly, overexpression resulted exhibit an inhibiting effect. Moreover, bioinformatics tool applied predict downstream target LINC00963. We investigated suppressed α-SMA induced TGF-β1 fibroblasts, at least part, downregulating expression miR-143-3p. addition, either promotion or miR-143-3p inhibition could significantly decrease myofibroblast contractility collagen I III secretion, which are contribute Taken together, our study promising therapeutic target.