Mycobacterium tuberculosis PE8 (Rv1040c) Promotes the Intracellular Survival of Recombinant Mycobacterium by Regulating Host Inflammatory Cytokines and Inhibiting Cell Late Apoptosis

• DOI: 10.1089/dna.2022.0316
• Publication Date: 2023-04-19T13:35:21Z
• Authors: Tao Xu; Chutong Wang; Minying Li; Meili Yuan; Jing Wei; Baiqing Li; Zhongqing Qian; Ting Wang; Xiaojing Wang; Hongtao Wang
• Keywords: Mycobacterium smegmatis; Proinflammatory cytokine; Virulence factor; Intracellular parasite; Cell envelope

Tuberculosis is an important chronic and often fatal infectious disease mainly caused by the bacterium Mycobacterium tuberculosis (Mtb). Mtb one of most successful pathogens that harbors several potential virulence factors not found in nonpathogenic mycobacteria. As cell envelope closely associated with its resistance, it very to understand for better treatment causative pathogen. There increasing evidence Pro-Glu (PE) Pro-Pro-Glu (PPE) proteins are major effectors persistence encoded H37Rv genome. However, function PE8 has been explored date. In this study, we heterologously expressed nonpathogenic, fast-growing M. smegmatis investigate interaction between host determine possible biological functions. We recombinant cells expressing were less susceptible sodium dodecyl sulfate-induced surface stress compared those empty vector, suggesting may be involved responses. addition, macrophages infected PE8-expressing produced obviously lower levels proinflammatory factor IL-1β, IL-6, TNF-α higher inhibitory IL-10. further promoted survival within inhibiting late apoptosis macrophages. Collectively, selective targeting PE/PPE protein family offers untapped opportunity development more effective safer drugs against infection.