Gene Transfer in Regenerating Muscle

• DOI: 10.1089/hum.1994.5.1-11
• Publication Date: 2008-03-19T22:29:16Z
• Authors: Maurizio Vitadello; Maria Vittoria Sc...; Anne Picard; Maurizio Scarpa; Stefano Schiaffino
• Keywords: Gene transfer

We have compared the efficiency of direct gene transfer in normal and regenerating rat skeletal muscle. Muscle necrosis regeneration was induced by intramuscular injection bupivacaine soleus muscle adult rats. Plasmids containing β-galactosidase (β-gal) or chloramphenicol acetyltransferase (CAT) genes driven viral promoters were injected 3 days after treatment into contralateral uninjured muscles. Expression CAT activity >80-fold higher to control muscles at 7 post-transfection, but decreased 30 60 days. Southern blot analysis showed that predominant form DNA episomal transfected muscles; however, measurements performed on same no precise correlation between enzymatic amount plasmid DNA. β-gal detected numerous fibers injured post-transfection; contrast, only rare positive found Focal infiltrates mononuclear cells, which surround invade selectively β-gal-positive fiber segments, observed suggesting immune mechanisms are implicated progressive loss transgenes with time. The finding display a transfection may be relevant therapy Duchenne muscular dystrophy, because early stages disease. Skeletal can vivo DNA; is relatively low. Vitadello et al. show leads protein expression levels result since abundant