Clonal and Plasmid-Mediated Dissemination of β-Lactamases Producing Klebsiella spp. Among Environment and Humans in an Intensive Vegetable Cultivation Area in Eastern China

• DOI: 10.1089/mdr.2024.0140
• Publication Date: 2025-04-14T16:36:01Z
• Authors: Kaixin Jiang; Yunting Jiang; Qian Zhao; Zhenhua Shang; Huiyun Zou; Jiliang Si; Tianle Wu; Xuewen Li
• Keywords: Klebsiella; Klebsiella infections

Bckground: Emergence and the rising prevalence of extended-spectrum β-lactamases (ESBLs) producing multidrug-resistant Klebsiella spp. is a global concern. Methods: 391 samples were collected from environmental people in an intensive vegetable cultivation area eastern China June 2019. ESBLs-producing obtained by PCR strain identification. The resistance genotype phenotype determined drug susceptibility test. number size plasmids pulsed-field gel electrophoresis assays plasmids. plasmid blaCTX-M was DNA imprinting hybridization, transferability understood conjugation experiment. Whole-genome sequencing analysis (WGS) used to obtain other antimicrobial genes, virulence factors, mobile elements, genetic environment. Results: Seventeen ESBL-producing multi-drug resistant. Sixteen carried blaCTX-M, containing anged ∼33.3 kb ∼244.4 kb. Thirteen successfully transferred recipient bacterium through mediation. Single nucleotide polymorphism showed clonal transmission between river water (J4-J8) sediment (J9), (J3) human feces (J12). WGS that all associated with element Tn3 and/or IS1380 family. All strains factors related adhesion, colonization, pathogenicity. Conclusion: This study reminds us antibiotic-resistant bacteria (ARB) environments can spread human. It vital enhance surveillance high vigilance for risk ARB movement plantation environment humans.