[18F]FDG-PET Combined with MRI Elucidates the Pathophysiology of Traumatic Brain Injury in Rats

• DOI: 10.1089/neu.2016.4540
• Publication Date: 2016-08-24T07:43:40Z
• Authors: Fiona Brabazon; Colin M. Wilson; Dinesh K Shukla; Sanjeev Mathur; Shalini Jaiswal; Sara Bermudez; Kimberly R. Byrnes; Reed Selwyn
• Keywords: Male; Amygdala; Hippocampus; Magnetic Resonance Imaging; Corpus Callosum; Rats; Rats, Sprague-Dawley; Disease Models, Animal; 03 medical and health sciences; 0302 clinical medicine; Fluorodeoxyglucose F18; Positron-Emission Tomography; Brain Injuries, Traumatic; Animals; Microglia; Radiopharmaceuticals

Non-invasive measurements of brain metabolism using 18F-fluorodeoxyglucose (FDG) with positron emission tomography (PET) may provide important information about injury severity following traumatic (TBI). There is growing interest in the potential combining functional PET imaging anatomical and magnetic resonance (MRI). This study aimed to investigate effectiveness clinically available FDG-PET T2 diffusion MR imaging, a particular focus on inflammation influence glial alterations after injury. Adult male Sprague Dawley rats underwent moderate controlled cortical impact (CCI) followed by FDG-PET, MRI, histological evaluation. FDG uptake showed significant corpus callosum, hippocampus, amygdala TBI, demonstrating that relatively "focal" CCI can result global alterations. Analysis MRI intensity apparent coefficient (ADC) also these regions include cytotoxic vasogenic edema. Histology increased activation callosum hippocampus was associated at sub-acute time-points. Glial not detected but neuronal damage evident, as only region show reduction both ADC Overall, confounded presence cell damage, whereas consistently activation. These results demonstrate be used together improve our understanding complex TBI.