Plasma Neurofilament Light and Glial Fibrillary Acidic Protein Levels over Thirty Days in a Porcine Model of Traumatic Brain Injury

• DOI: 10.1089/neu.2022.0070
• Publication Date: 2022-04-04T07:02:52Z
• Authors: Samuel S. Shin; Marco M. Hefti; Vanessa M. Mazandi; David A. Issadore; David F. Meaney; Andrea L. C. Schn...; Ramon Diaz-Arrastia; Todd J. Kilbaugh
• Keywords: Corona radiata (embryology); Radiata; Neurofilament; Diffuse axonal injury

To establish the clinical relevance of porcine model traumatic brain injury (TBI) using plasma biomarkers with diffusion tensor imaging (DTI) over 30 days, we performed a randomized, blinded, pre-clinical trial Yorkshire pigs weighing 7-10 kg. Twelve were subjected to Sham (n = 5) by skin incision or TBI 7) controlled cortical impact. Blood samples collected before injury, then at approximately 5-day intervals until days. Both groups also had DTI 24 h and days after injury. Plasma isolated single molecule array (Simoa) was for glial fibrillary acidic protein (GFAP) neurofilament light (NFL) levels. Afterwards, tissue stained β-APP. showed fractional anisotropy (FA) decrease in right corona radiata (ipsilateral injury), contralateral radiata, anterior corpus callosum 1 day. At ipsilateral decreased FA. Pigs increase GFAP NFL 1-5 Significant difference between animals continued up 20 Linear regression significant negative correlation FA both levels further validate degree axonal found DTI, β-APP immunohistochemistry on perilesional as well bilaterally. Variable staining radiata. Porcine replicates acute seen TBI. Further, long term white matter is confirmed areas such splenium However, future study stratifying severe mild TBI, comparison other subtypes diffuse may be warranted.