The Effects of Helium-Neon Light Therapy on Healing of Partial Osteotomy of the Tibia in Streptozotocin Induced Diabetic Rats

• DOI: 10.1089/pho.2008.2421
• Publication Date: 2009-12-27T00:46:34Z
• Authors: Shabnam Abdi; Mohammad Bayat; Farshad Javadieh; Zhaleh Mohsenifar; Fatemesadat Rezaie; Maryam Bayat
• Keywords: Fracture Healing; Male; Analysis of Variance; Tibia; Neon; Helium; Statistics, Nonparametric; Streptozocin; Diabetes Mellitus, Experimental; Osteotomy; Rats; 3. Good health; Tibial Fractures; Disease Models, Animal; 03 medical and health sciences; 0302 clinical medicine; Animals; Stress, Mechanical; Low-Level Light Therapy; Rats, Wistar

The effect of light therapy (LT) on surgically created partial osteotomy in streptozotocin (STZ)-induced diabetic rats was examined.LT has been shown to enhance bone repair in healthy human and animal models.Forty male rats were divided into groups 1 to 5. Diabetes was induced in rats of groups 1, 2, and 3 using an intraperitoneal injection of STZ. All diabetic rats were maintained for 30 days after STZ injection. Under general anesthesia and sterile conditions, a partial transversal standardized osteotomy was made in the mid-portion of the right tibia. The defects in groups 2, 3, and 5 were treated using a helium-neon (He-Ne) laser (632.8 nm, 10 mW, circular beam shape). Groups 1 and 4 were diabetic placebo and normal placebo groups, respectively. A dose of 369.4 J/cm2 for groups 2 and 5 and a dose of 66.8 J/cm2 for group 3 were applied three times a week. Six weeks after surgery, the right tibia was collected. The specimen was subjected to a three-point bending test.LT with 369.4 J/cm2 energy density resulted in significantly greater bending stiffness in group 5 (41.8+/-5.2) than in groups 1 (18.5+/-4.1), 2 (17.7+/-1.6), and 3 (11.5+/-4) (least significant difference (LSD) test, p<0.01, p<0.001, and p<0.001, respectively). LT with 369.4 J/cm2 energy density resulted in a significantly higher stress load in group 5 (10+/-0.4) than in groups 1 (4.9+/-1.5), 2 (5.7+/-0.52), and 3 (3.9+/-1.1) (LSD test, p<0.01, p<0.01, p<0.001, respectively).LT with a He-Ne laser in STZ-induced diabetic rats did not enhance bone repair of a partial transversal standardized osteotomy.