Ovariectomy-Induced Osteopenia Influences the Middle and Late Periods of Bone Healing in a Mouse Femoral Osteotomy Model

• DOI: 10.1089/rej.2015.1682
• Publication Date: 2015-02-19T10:51:51Z
• Authors: Jian Pang; Meina Ye; Xinfeng Gu; Yuelong Cao; Yuxin Zheng; Hailing Guo; Yongfang Zhao; Hongsheng Zhan; Yinyu Shi
• Keywords: Osteopenia; Femoral fracture

Objective: It is known that bone healing delayed in the presence of osteoporosis humans. However, due to complexities osteoporotic fractures, animal models may be more appropriate for studying effects detail and testing drugs on fracture repair process. The purpose this study was investigate influence ovariectomy-induced osteopenia an open femoral osteotomy model, test feasibility model evaluating process under osteopenic conditions. Methods: Ovariectomized (OVX) mouse were employed assess healing, A mid-shaft femur also established 3 weeks after ovariectomy as group (OVX group). Femurs then harvested at 2 6 X-ray radiography, micro-computed tomography (micro-CT), histology, biomechanical analysis. sham-operated (sham group) used comparison. Results: OVX mice had significantly lower volume density (BVF), volumetric mineral (vBMD), tissue (TMD) calluses (p<0.05), similar trend observed weeks. Additionally, larger animals via micro-CT X-ray, but these did not result better outcomes, determined by Histological images fractures found hastening broken end resorption delay hard callus remodeling. impaired measurements (p<0.05) consistent with radiographic scoring, which indicated group. Conclusions: This provided evidence impair middle late These data supported validity