Comprehensive Phenotypic Characterization of Human Adipose-Derived Stromal/Stem Cells and Their Subsets by a High Throughput Technology

• DOI: 10.1089/scd.2012.0346
• Publication Date: 2012-08-24T18:10:44Z
• Authors: Patrick C. Baer; Selim Kuçi; Michael Krause; Zyrafete Kuçi; Stefan Zielen; Helmut Geiger; Peter Bader; Ralf Schubert
• Keywords: CD90; Immunophenotyping; Stem cell marker

The characterization of adipose-derived stromal/stem cells (ASCs) remains difficult due to the lack a definitive and unique cellular marker. Therefore, combination markers is necessary identify cells. No comprehensive analysis immunophenotype expanded plastic adherent ASCs has been published. aim this study was characterize general phenotype cultured further analyze subsets. were isolated from lipoaspirates patients undergoing cosmetic liposuction in standard cell culture. A done with BD Lyoplate™ Human Cell Surface Marker Screening Panel containing 242 antibodies isotype controls. Cultured not only showed characteristic expression profile mesenchymal stem (MSCs), but also revealed donor-specific variability 49 other markers. We detected scattering fluorescence intensity, indicating subpopulations different profiles. multi-color flow cytometric after staining direct-labeled against CD73, CD90, CD105, either CD34, CD140b, CD200, CD201, or CD36 verify selected ASCs. no CD34-CD36 double-positive population, CD34(+)-CD36(-) CD34(-)CD36(+) subpopulations, both which are positive for 3 main MSC markers, CD105. All co-expressed therefore fulfill minimal phenotypic criteria definition MSCs. Our demonstrates first clearly highlights ASC preparations.