Functional Differences in the Role of Ductal Stem Cells in Mouse Major Salivary Glands

• DOI: 10.1089/scd.2022.0266
• Publication Date: 2022-12-21T11:02:57Z
• Authors: Raksha Narendra; Ninche Ninche; Soosan Ghazizadeh
• Keywords: Ductal cells; Keratin 14

Salivary gland (SG) stem cells are the only cell population capable of extended growth in organotypic cultures, and thus they considered a source for cell-based therapies aimed at SG regeneration. Studies mouse submandibular have identified one tissue salisphere formation culture. These actively dividing ductal that express epithelial progenitor markers keratin (K) 5/14 normally function as lineage-restricted differentiated cells. In response to severe injury, however, these undergo multipotency switch contribute regeneration multiple lineages, including secretory units or acini. Little is known about mechanism renewal other major SGs whether comparable populations exist parotid (PG) sublingual (SLG) glands. Using vivo ex models, we show both PG SLG contain small K14-expressing Although do not cycle frequently, salisphere-forming Long-term lineage tracing studies adult PGs showed progenitor-progeny relationship between K19-expressing striated ducts. SLGs, did generate progeny 6-month period observation make significant contribution after injury. reveal functional similarities differences among implications developing strategies regenerative therapies.