3D Printed Platelet-Rich Plasma-Loaded Scaffold with Sustained Cytokine Release for Bone Defect Repair

• DOI: 10.1089/ten.tea.2021.0211
• Publication Date: 2022-02-12T12:28:48Z
• Authors: Chun Liu; Ziyue Peng; Haixia Xu; Huiling Gao; Jianjun Li; Yanglei Jin; Yihan Wang; Chengqiang Wang; Yang Liu; Yunteng Hu; Cong Jiang; Jiasong Guo; Lixin Zhu
• Keywords: Polycaprolactone; Gelatin

The combination of three-dimensional (3D) printed scaffold materials and various cytokines can achieve the purpose tissue reconstruction more efficiently. In this study, we prepared platelet-rich plasma (PRP)/gelatin microspheres combined with 3D polycaprolactone/β-tricalcium phosphate scaffolds to solve key problem that PRP cannot be released under control release time is too short, thus better promote bone repair. Consequently, composite displayed a good mechanical property sustained cytokine for ∼3 weeks. Increased survival, proliferation, migration, osteogenic angiogenic differentiation marrow mesenchymal stem cells were observed compared groups. in vivo study demonstrated PRP/gelatin led greater positive effects promoting large defect conclusion, new type long-term sustained-release material was constructed effectively improved transplanted area, thereby repair defects. Impact statement Reconstruction blood vessels at defects takes time. Platelet-rich (PRP) has been widely used because it contains variety local osteogenesis angiogenesis. phosphate/PRP/gelatin predicament short PRP-related materials. We proved not only (more than 3 weeks) but also believe novel concept developing releasing bioscaffold treating defect.