Dengue Infection in Neotropical Forest Mammals

• DOI: 10.1089/vbz.2007.0280
• Publication Date: 2008-10-22T19:57:38Z
• Authors: Benoît de Thoisy; Vincent Lacoste; Adeline Germain; Jorge Muñoz-Jordán; Candimar Colón; Jean-François Mau...; Marguerite Delaval; François Catzeflis; Mirdad Kazanji; Séverine Matheus; Philippe Dussart; Jacques Morvan; Alvaro Aguilar Se...; Xavier Deparis; Anne Lavergne
• Keywords: Flavivirus

In South America, dengue is the arbovirus-transmitted disease with highest incidence. Unlike other arboviruses, wild mammals have no confirmed role in cycle of neotropics, although serological studies suggested a possible secondary amplification involving than nonhuman primates. French Guiana, where all four serotypes (DENV-1, DENV-2, DENV-3, DENV-4) are present, endemic outbreak events. To determine whether can be infected by DENV, rodents, marsupials, and bats were captured over several periods, from 2001 to 2007, at two sites. The first location forest surrounded an urban area endemic. second edge site has not yet emerged. A total 10,000 trap-nights performed 616 captured. RNAs representing DENV detected both sites reverse-transcriptase polymerase chain reaction livers and/or sera 92 belonging 14 out 32 species distributed among orders investigated: Rodentia (33 positive/146 tested), Marsupialia (40/318), Chiroptera (19/152). Sequence analyses portion capsid premembrane junction revealed that mammal strains DENV-1, DENV-4 had only 92.6%, 89%, 95%, 95.8% identity, respectively, circulating human population during same periods. Regarding related (99% identity) those responsible for epidemic event humans Guiana concurrent capture sessions also evidenced, suggesting habitats strains. Our results demonstrate, time, neotropical virus. question maintain enzootic cycles play its reemergence populations remains answered.