Dynamin Participates in Focal Extracellular Matrix Degradation by Invasive Cells

• DOI: 10.1091/mbc.e02-05-0308
• Publication Date: 2003-03-11T22:44:48Z
• Authors: Massimiliano Bald...; Arsenio Pompeo; Galina Beznoussenko; Claudia Castaldi; Salvatore Cortellino; Mark A. McNiven; Alberto Luini; Roberto Buccione
• Keywords: Invadopodia

The degradation of extracellular matrix (ECM) by metalloproteases is crucial in physiological and pathological cell invasion alike. Degradation occurs at specific sites where invasive cells make contact with the ECM via specialized plasma membrane protrusions termed invadopodia. Herein, we show that dynamin 2 (Dyn2), a GTPase implicated control actin-driven cytoskeletal remodeling events transport, necessary for focalized Dynamin was inhibited using two approaches: 1) expression dominant negative GTPase-impaired or proline-rich domain-deleted Dyn2 mutants; 2) inhibition regulator calcineurin cyclosporin A. In both cases, number extension foci were drastically reduced. To understand site mechanism action, cellular structures devoted to analyzed correlative confocal light-electron microscopy. Invadopodia found be organized into previously undescribed ECM-degradation structure consisting large invagination ventral surface close spatial relationship Golgi complex. seemed concentrated