Interactions between the Evolutionarily Conserved, Actin-related Protein, Arp11, Actin, and Arp1

• DOI: 10.1091/mbc.e03-01-0049
• Publication Date: 2003-03-25T01:46:37Z
• Authors: D. Mark Eckley; Trina A. Schroer
• Keywords: Dynactin; Actin-binding protein; MDia1

The dynein activator dynactin is a multiprotein complex with distinct microtubule- and cargo-binding domains. domain contains short, actin-like filament of the actin-related protein Arp1, second protein, Arp11, conventional actin. length this invariant in isolated from multiple species tissues, suggesting that activities regulate Arp1 polymerization are important for assembly. Arp11 present localized at pointed end minifilament, whereas actin capping (CapZ) barbed end. Either might cooperate to cap Arp1. We tested ability interact found it could coassemble. Like cytosolic only dynactin, free do not significantly. Recombinant were demonstrated by coprecipitation. developed an vivo assay Arp11-Arp1 interaction based on previous observations forms filamentous assemblies when overexpressed cultured cells. significantly decreases formation these organized assemblies. Finally, was used confirm identity putative homolog Drosophila melanogaster.