Golgi Enzymes Are Enriched in Perforated Zones of Golgi Cisternae but Are Depleted in COPI Vesicles

• DOI: 10.1091/mbc.e03-12-0881
• Publication Date: 2004-07-29T00:15:00Z
• Authors: Hee-Seok Kweon; Galina V. Beznous...; Massimo Micaroni; Roman S. Polishchuk; Alvar Trucco; Oliviano Martella; Daniele Di Giando...; Pierfrancesco Marra; Aurora Fusella; Alessio Di Pentima; Eric G. Berger; Willie J. C. Geerts; Abraham J. Koster; Koert N. J. Burger; Alberto Luini; Alexander A. Mironov
• Keywords: COPI; COPII

In the most widely accepted version of cisternal maturation/progression model intra-Golgi transport, polarity Golgi complex is maintained by retrograde transport enzymes in COPI-coated vesicles. By analyzing enzyme localization relation to three-dimensional ultrastructure complex, we now observe that are depleted buds and 50- 60-nm COPI-dependent vesicles a variety different cell types. Instead, find concentrated perforated zones rims both vivo cell-free system. This lateral segregation detectable some stacks during steady-state but it was significantly prominent after blocking endoplasmic reticulum-to-Golgi transport. Delivery carriers release block leads diminution concentrations cisternae. The exclusion from COPI their transport-dependent accumulation argues against current vesicle-mediated model.