Role of Numb in Dendritic Spine Development with a Cdc42 GEF Intersectin and EphB2

• DOI: 10.1091/mbc.e05-07-0700
• Publication Date: 2006-01-05T02:18:20Z
• Authors: Takashi Nishimura; Tomoya Yamaguchi; Akinori Tokunaga; Akitoshi Hara; Tomonari Hamaguchi; Katsuhiro Kato; Akihiro Iwamatsu; Hideyuki Okano; Kozo Kaibuchi
• Keywords: NUMB

Numb has been implicated in cortical neurogenesis during nervous system development, as a result of its asymmetric partitioning and antagonizing Notch signaling. Recent studies have revealed that functions clathrin-dependent endocytosis by binding to the AP-2 complex. is also expressed postmitotic neurons plays role axonal growth. However, later stages neuronal development remain unknown. Here, we report specifically localizes dendritic spines cultured hippocampal spine morphogenesis, partially through direct interaction with intersectin, Cdc42 guanine nucleotide exchange factor (GEF). Intersectin multidomain adaptor for proteins involved cytoskeletal regulation. enhanced GEF activity intersectin toward vivo. Expression or caused elongation neck, whereas knockdown Numb-like decreased protrusion density length. Furthermore, formed complex EphB2 receptor-type tyrosine kinase NMDA-type glutamate receptors. Knockdown suppressed ephrin-B1-induced maturation. These results highlight synaptic EphB2.