Nup153 Affects Entry of Messenger and Ribosomal Ribonucleoproteins into the Nuclear Basket during Export

• DOI: 10.1091/mbc.e05-08-0715
• Publication Date: 2005-09-30T00:24:17Z
• Authors: Teresa Soop; Birgitta Ivarsson; Birgitta Björkroth; Nathalie Fomproix; Sergej Masich; Volker C. Cordes; Bertil Daneholt
• Keywords: Nuclear pore; Nucleoporin; Nucleoprotein; Messenger RNP; Bacteriophage MS2; Heterogeneous nuclear ribonucleoprotein

A specific messenger ribonucleoprotein (RNP) particle, Balbiani ring (BR) granules in the dipteran Chironomus tentans, can be visualized during passage through nuclear pore complex (NPC). We have now examined transport basket preceding actual translocation NPC. The consists of eight fibrils anchored to NPC core by nucleoprotein Nup153. On injection anti-Nup153, BR is blocked. Many are retained on top basket, whereas no seen transit Interestingly, effect Nup153 seems distant from antibody-binding site at base basket. conclude that entry into a two-step process: an mRMP first binds tip and only then it transferred Nup153-dependent process. It indicated ribosomal subunits follow similar pathway.